Ikaros induces the formation of multipolar neurons during cortical development


Bech Struck, Xavier


Ikaros belongs to a family of zinc finger transcription factors that modulate the differentiation process of the hematopoietic system during development. It is also expressed in the nervous system, where it plays a role in the identity specification of migrating neurons in the cortex, as well as the differentiation of a population of striatal neurons that express the Dopamine receptor-2 (D2R). However, these functions direct neuronal behaviour and the molecular mechanism involved remain largely unclear. Here we use in utero electroporation and cell based assays to reveal the role of Ikaros on migrating neurons. We show that Ikaros affects the migration, but not differentiation, of cortical neurons. Ikaros gain-of-function experiments show alterations in cell morphology and induction of multipolar morphology in cortical neurons, which in turn, reduces migration speed. Finally, we show that the most upregulated gene after Ikaros overexpression, Midkine, could modulate neuronal migration by inducing repulsion to migrating cortical neurons. Taken together, these results suggest that Ikaros controls radial migration by modulating multipolar morphology.



Carnicer Heras, Marc
Canals, Josep M.


IQS SE - Undergraduate Program in Biotechnology