Bioremediació d’aigües subterrànies per tractament enzimàtic

Author

Casademont Perramon, Maria

Abstract

This Project is focused on the study of environmental pollution issues and its current treatments. To test the efficiency of enzymatic catalysis for small scale degradation of hydrocarbons and their additives, one of the main environmental pollutants, an experimental design was carried out. For this purpose, three enzymatic products have been tested: Petra-zyme, a commercial enzyme based liquid mixture, and two laccases, with and without activator. The matrix is groundwater polluted with aromatic and aliphatic hydrocarbons, BTEX and the additives MTBE and ETBE. The assay is carried out at sample’s natural conditions and under agitation during 24 hours at different concentrations of the products.
No product significantly degraded the pollutants, although Petra-zyme at 6% (v/v) concentration degraded all pollutants below the limits of quantification. Laccase with integrated activator at a concentration of 3 U/mL reduced below the limit of quantification the volatile hydrocarbons of 5 to 6 carbons. Laccase with integrated activator at a concentration of 6 U/mL slightly reduced the concentration of o-xylene, MTBE and ETBE and, below the limit of quantification, the volatile hydrocarbons of 5 to 6 carbons. Finally, laccase without integrated activator at a concentration of 1U/mL slightly reduced the concentration of benzene, toluene, o-xylene, m, p-xylene, volatile TPH with more than 8-10 carbons, MTBE and ETBE.

Director

Planas Sauter, Antoni
Mora Vilamanyà, Ricard

Degree

IQS SE - Undergraduate Program in Biotechnology

Date

2022-06-18