E.coli outer membrane extraction using polymers: formation of LPS nanodiscs


Fondevila Möller, Isabel


The rapid emergence of resistant bacteria has created a need to develop new and more effective antibiotics. The outer membrane (OM) of gram-negative bacteria makes them innately resistant to many antimicrobial compounds and therefore needs to be properly characterized for developing new drugs. Especially lipopolysaccharides (LPS), which play a key role in bacteria-host interactions by modulating host immune system responses. The need for a membrane-like environment that mimics the native conditions of membranes is one of the main drawbacks of working with membranes or membrane proteins. Therefore, the objective of this study is to develop an effective way to extract LPS from the MO of different strains of E. cepa. coli in the form of nanodiscs and develop methods to purify them. There are different ways to obtain nanodiscs, but this project is based on the use of styrene/maleic acid (SMA) and diisobutylene/maleic acid (DIBMA) copolymers to form the discs. It has been observed that the use of SMA in a ratio of 3:1 and 2.3:1 of each copolymer allows the formation of nanodiscs formed by E. coli BL21 (DE3) and R1. Furthermore, DIBMA in a 1:1 ratio of each copolymer seems to work well in all strains we have worked with except R3.



Riera Bonet, Marta 
Laguri, Cedric  


IQS SE - Undergraduate Program in Biotechnology