Estudi de la modificació de gelatina pel desenvolupament d’scaffolds per la reconstrucció del cartílag

Author

Querol Duaso, Anna

Abstract

Currently there is no curative therapy for joint cartilage problems, nor for osteoarthritis or also related to osteoarthritis. Patients who suffer from this malady have symptoms of pain, inflammation, stiffness and blockage of the joint during their day to day life. Depending on the extent of the injury, the patient may be disabled for a long time to live a quality lifestyle. For this reason, the objective of this TFG is to be able to make an advance in the field of biomaterials for the development of a scaffold for the reconstruction of cartilage through cellular therapy. The idea is to be able to form a chemically modified gelatin hydrogel of porcine origin. Gelatin is a product that has properties similar to the human extracellular matrix, but by itself it cannot form a hydrogel. For this reason, mixing the anhydride methacrylic forms the GelMA hydrogel, and this can consequently be printed in 3D as the structure of the suitable scaffold.
To formulate these biodyes, a methodology is first developed to study and calculate the different methacrylate levels of GelMA. Next, it is determined which factors most influence the DoF and analyze how the DoF of GelMA affects cell viability, osmolarity, pH and viscosity. Lastly, GelMA is synthesized with human gelatin extract (SoHO) reproduced under conditions of 100% methacrylation.

Director

Texidó Bartés, Robert

Degree

IQS SE - Undergraduate Program in Chemistry

Date

2022-06-18