Experimental validation of candidate effector genes as targets for boosting titers and productivity of therapeutic proteins in CHO cells


Rueda Matas, Ariana


Biopharmaceuticals such as recombinant proteins have been on the rise in recent years due to their ability to treat a wide range of complex diseases. The preferred recombinant host for producing these drugs is CHO cells, a cell line known for its ability to emulate human post-transnational modifications. As the production platform for most of the best-selling drugs in the industry, a lot of effort has gone into optimizing the performance of these cells. However, CHO cells still have considerably low productivity compared to other recombinant hosts. Taking advantage of the large amount of data available in public resources, a computational analysis was implemented to find genes with the potential to improve the secretory capacity of CHO cells. In this project, the experimental validation of a set of genes will be carried out using two parental cell lines previously designed to express the recombinant model proteins Erythropoietin and Etanercept.



Camacho Zaragoza, Jose Manuel
Carnicer Heras, Marc  


IQS SE - Undergraduate Program in Biotechnology