Hidrogeles entrecruzados para fijación enzimática en biosensores amperométricos de glucosa


Danilo Fortin, Jose


Electrochemical biosensors are analytical devices that have emerged as a promising alternative to traditional analytical techniques, due to they combine the robustness of electrochemical analysis techniques with the specificity of biological recognition processes, therefore offering great advantages such as size, sensitivity, selectivity and fast response. Because of these qualities, electrochemical biosensors have many applications in food, pharmaceutical, clinical and healthcare industries. In this work, crosslinked hydrogels of chitosan/PEGDA and HEMA/EGDA have been synthesized by UV photopolymerization using a radical initiator (Irgacure 2959). In order to obtain these crosslinked hydrogels, several tests were carried out at different proportions of reagents, with the aim of achieving hydrogels that were stable and suitable to be used as an immobilizer material for glucose oxidase enzyme (GOx). Subsequently, enzymatic amperometric biosensors to measure glucose in solution were developed using HEMA/EGDA and chitosan hydrogels as immobilizing matrices. Glucose oxidase enzyme (GOx) was immobilized on an electrochemical interface of highly ordered titanium oxide (IV) nanotubes (TiO₂NTs) prepared for each biosensor to be constructed.
Biosensors developed with these immobilizing matrices were evaluated by studying their sensitivity and long-term stability through amperometric  measurements of glucose in solution.



Colominas Fuster, Sergi
Abellà Iglesias, Jordi  


IQS SE - Master’s Degree in Materials Science and Engineering