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Author
Cardona i Vilumara, Vicenç
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Abstract
The production of stem cells with the use of microcarrier technology has made it possible to meet the demand for the number of cells required in certain clinical applications within cell therapy. Although scaling processes at an industrial level are simpler with this technology compared to traditional crops (2D surfaces), although certain stages of the upstream, downstream and storage process must be optimized.
The proteolytic enzymes that are used during the harvesting of the cells, separating them from the growth surface of the microcarriers, make the process not robust, and it entails a significant economic cost. For this reason, to optimize the scaling process, a search has been made for different pH-sensitive polymers that allow the instant release of cells from their growth surface without the need for proteolytic enzymes. With the choice of PAAC, NIPAAm, DMAAm, NEAAm and PEI polymers, two different ways have been proposed to synthesize pH-sensitive microcarriers with a diameter size between the values of 120 to 200 microns that allow this cellular release at pH values between 7.7 and 8. the first synthesis proposal is through the emulsion polymerization method that allows the synthesis of microcarrier depending on the concentration of surfactants in the culture medium and the second is by grafting these polymers on the surface of the commercial microcarrier Cytodex 3.
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