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Author
Font Moré, Mercè
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Abstract
In this final degree project, a single-use bioreactor is designed and built using a Wave bioreactor system. In addition, this project studies the effect of the mixing parameters on the gas transfer regime that consequently impact on the oxygen available to the culture inside of the reactor. Finally, the progress of a culture of Pichia pastoris within the developed bioreactor is studied and this is compared with a culture that progresses in a shaker.
The results obtained indicate that the developed bioreactor is suitable to support a culture. The most favorable transfer regime conditions for the culture will occur when there is more mixing in the bioreactor (16 rpm rocking speed and 10º angle). From the comparison of the two cultures using in one the developed disposable bioreactor and in the other a shaker, it is observed how using the previously studied mixing conditions the two cultures have a very similar growth.
It has also been possible to monitor the culture inside the single-use reactor in two different ways. On the one hand, extracting samples periodically from inside the reactor, and on the other, using a BlueSence sensor it has been possible to know the oxygen concentration at the gas outlet of the reactor and finally calculate the rate of oxygen absorption.
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