Oxygen mapping in cells using genetically-encodable fluorescent proteins


Ruiz Pérez, Teresa


Photodynamic therapy is a cancer treatment that combines drugs, light and oxygen to selectively kill cancer cells. Cytotoxic reactive oxygen species are produced, which oxidize key cellular components and thus trigger cell death mechanisms. For successful therapy, good oxygenation of the tumour is essential, so it is necessary to monitor, in real-time, the oxygen concentration in the cells. Genetically encoded fluorescent proteins, in which a chromophore is protected from the environment inside the protein, have lifetimes in the excited state that are very sensitive to oxygen and could therefore be used for photodynamic therapy. The project aims to express fluorescent flavin-binding proteins in bacteria (E.coli) and to characterize the photochemistry characteristics of the pure protein. An expression and purification methodology has been established to purify DsFbFPM49I (our protein of interest) to a concentration of 0.15 mg/L culture.



Faijes Simona, Magda
Nonell Marrugat, Santi


IQS SE - Undergraduate Program in Biotechnology