The role of Ikaros in multipolar identity in the developing striatum


Zarzuela Alonso, Xavier


Confirming the presence of multipolar neurons in the development stages in the striatum, as well as observing how Ikaros is involved in this process, is the objective of this project. The following work plan is proposed:

1.) Identify multipolar neurons in the striatum. GFP immunohistochemistry in in utero electroporated (SUI) brains. Time-lapse imaging of GFP control brains brain slices to observe multipolar behavior.

2.) Observe the effect of Ikaros on multipolar neurons in the striatum. Design of the Ikaros-IRES-GFP construct. GFP IHC and time-lapse imaging in Ikaros-IRES-GFP SUI brains to study how Ikaros expression affects multipolar neuron number and behavior in the developing striatum.

3.) Ex vivo experiments. To observe the behavior of MPN ex vivo using a system of nanofibers of radial glia and striatal explants of IUE GFP and Ikaros-IRES-GFP brains.



Fornaguera Puigvert, Cristina


IQS SE - Undergraduate Program in Biotechnology