Structure of Chitin Deacetylases. Dicovery anc characterization of novel Enzymes


Rodriguez Herrera, Daniel


Chitin is the second most abundant amino polysaccharide and the second most abundant organic molecule after cellulose due to its wide distribution in various organisms, from arthropods to diatoms. The depolymerization and N-acetylation of chitin and its monomers (COS) for chitinase and chitin deacetylases (CDA) participate in molecular recognition events such as modulation of cell signaling and morphogenesis, immune responses and host-pathogen interactions. Chitosan, the deacetylated form of chitin, and COS are also attractive scaffolds for the development of bionanomaterials for drug / gene delivery and tissue engineering applications. Most of the biological activities associated with BODY appear to depend largely on the degree of polymerization and the acetylation pattern, which defines the charge density and distribution of GlcNAc and GlcNH2 parts in chitosan and COS. studies of new ADCs with their own characteristics that serve as a tool to better understand the mechanism involved in their functions. Therefore, this project is intended to be a brief review of current knowledge of bacterial, fungal and insect ADCs, as well as a compilation of new ADCs that have been published since 2018. Furthermore, this project is intended to be a starting point in the expression and characterization of the unclassified ChbG protein, a chitin deacetylase from E. coli with the cloning of the ChbG gene into an expression vector.



Planas Sauter, Antoni 


IQS SE - Undergraduate Program in Biotechnology