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Author Torras Otaola, Josep Mª |
Abstract Previous research has shown how bacterial cellulose can improve the regeneration capability of plants with several genes being identified as possible candidates responsible for that enhancement. The objective of this work is to understand which of those genes have a bigger impact on this response. To do this, two kinds of experiences are carried out. One based on a reverse genetics approach where mutant seed lines where, one of the genes, has been eliminated to see the phenotypic impact that this has on the regenerative capacity of the plant when it has been treated with bacterial cellulose. Another one based on a genetic engineering approach where wildtype plants are being introduced (by means of Greengate technique) a fluorescent protein in the genes of interest to observe and quantify the response under the bacterial cellulose conditions. The results of the first experience points towards ELP as the main gene causing the enhancement, although the results are not conclusive and further research should be carried out. After finishing the transformations of the second experiments several mutant seeds where successfully obtained but the experiences with the bacterial cellulose couldn’t be finished on time. Thus this last experience should be carried on and finish in order to test whether the expression of the genes is related with the bacterial cellulose. |
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Director Leivar Rico, Pau |
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Degree IQS SE - Undergraduate Program in Biotechnology |
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Date 2021-09-07
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