Characterization of a novel Bruch’s membrane analogue based on decellularized tissue for the development of outer blood-retinal barrier models


Garrido Balcells, Patricia


Sight is one of the most important senses that we have to interact with the environment. Losing sight causes an enormous change in our daily life. However, there are many ophthalmologic diseases which may end in vision loss. The main causes of blindness are age-related macular degeneration and diabetic retinopathy which are related with the disruption of the outer blood retinal barrier (oBRB), which is composed by the retinal pigment epithelium, the Bruch’s membrane and the choriocapillaris. There is not a definitive treatment to end with oBRB-related diseases. Moreover, the treatments used have to be administrated periodically and these treatments are not patient friendly. More investigation is needed to develop more efficient treatments for oBRB related disease. The main inconvenient is the lack of proper models able to replicate the oBRB microenvironment. Therefore, the main objective of the present work is to develop a biomaterial that mimics the BM based on decellullarized BM tissue obtained from porcine eyes. In this project we have characterized this novel biomaterial and we have functionally compared it to other conventional protein coatings used for RPE culture.



Martorell López, Jordi
Molins, Blanca


IQS SE - Undergraduate Program in Biotechnology