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Author Querol Pérez, Gisela Carlota |
Abstract Glycoglycerolipids (GGLs) are a type of glycolipid abundant in photosynthetic organisms because of their importance in the light-harvesting system and mostly constitute the membranes of thylakoids and chloroplast envelopes. GGLs consist of an apolar component (diacylglycerol, DAG) and a polar component (usually one or two galactoses or glucoses). Their properties are of biotechnological interest in various applications, such as biosurfactants or drug delivery. The characterisation of GGLs in photosynthetic microorganisms, such as the microalga Chlamydomonas reinhardtii, is of great interest to study their fatty acid profile in response to changes in lipid metabolism in metabolic engineering experiments aimed at the production of glycolipids of interest. In this project, a method for the analysis of monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglycerols (DGDG) present in the polar lipid fraction has been developed. By means of total lipid extraction techniques, fractionation with thin layer chromatography and gas chromatography, it has been possible to develop an effective method for the analysis of the total fatty acid profile and in the MGDG and DGDG fraction. Furthermore, this method has been applied to detect changes in the fatty acid profile in strains genetically modified to produce mono-rhamnolipid biosurfactants, and the preparation of a construct for the expression of Mycoplasma genitalium glycosyltransferase MG517 in the chloroplast of microalgae has been initiated. |
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Director Planas Sauter, Antoni |
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Degree IQS SE - Master’s Degree in Bioengineering |
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Date 2022-07-09
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