Diseño de estrategias basadas en CRISPR/Cas9 para la generación de mutantes y su posterior validación experimental


González Navarro, Irene  


Since its discovery, the bacterial immune system known as CRISPR / CAS9 has established itself as one of the most favorable genetic editing tools for its versatility, ease of use and good results. The adaptation of the same for the genomic modification in eukaryotic organisms has supposed a big progress in the last years in allowing to generate models of diseases for the development of innovative drugs, as well as possibility to originate new knowledge on the gene regulation.
To understand gene regulation, it is of particular interest to know the role that mobile elements (TEs) play in the genome; these molecules correspond to DNA sequences that have the ability to change their position within the genetic material, thus being able to modify the structure and gene expression of itself, so that clarifying its function is key to to a greater understanding of the regulatory processes of organisms.
To all this, and in the absence of a test in which CRISPR technology has been applied to elucidate the effect of TEs in the model organism Drosophila melanogaster, in the present work two strategies based on CRISPR / CAS9 are proposed to achieve, on the one hand, the removal of the element FBti0019985 in the ISO-1 reference genome, with the aim of confirming that it is an adaptive mutation involved in cold and immune stress response processes; on the other, the interference of a specific family of TEs in the RAL-177 strain of D.melanogaster, to establish how it affects gene transcription after exposure to xenobiotics.
Keywords: Bacterial immune system, CRISPR / CAS9, adaptation, mobile elements, TEs, Drosophila melanogaster, elimination, FBti0019985, ISO-1, adaptive mutation, interference, family of TEs, RAL-177, xenobiotics.



Faijes Simona, Magda 


IQS SE - Undergraduate Program in Biotechnology