Estudi de medis de cultius pel cultiu d'hibridoma i la producció d'anticossos


Gelonch Capell, Paula  


The monoclonal antibodies products in the market have revolutionized the research and medical field, and currently it represents the half biopharmaceutical market products approximately. It is a market that is growing due to persistent innovation in the new drugs development and it is expected to continue doing so. The monoclonal antibodies production on a commercial scale is generally aimed at the diagnosis, therapy and new therapeutic agents research and development. Thus, this competitive industry is very strict with the cost, response time and regulatory requirements of the products. And now, the challenge is to develop and scale up purification processes capable of producing large quantities of purified monoclonal antibodies at the lowest possible cost.
In this study, different culture media that allow higher productivity of the murine monoclonal antibody IgG3 against human albumin have been evaluated by varying the concentrations of serum and other nutrients of various cell cultures of the mouse hybridoma 1-EAE. While the DMEM medium supplemented with only 10% of serum has not worked, the other two media tested (CD medium without serum and DMEM medium with a composition of 5% serum but with the presence of other supplements) have resulted in high cell growth and similar among them. The concentration of sodium bicarbonate necessary in the culture has also been evaluated for optimum pH buffering. To minimize aggressive pH conditions during the use of G protein purification columns (GE Healthcare HiTrap Protein G) for antibody purification, higher pH elutions have been examined to conserve the valuable monoclonal antibodies biological activity.

Key words: Immunoglobulins, monoclonal antibodies, hybridoma, culture medium, upstream, downstream, cell growth kinetics, buffer, sodium bicarbonate, purification.



Lecina Veciana, Martí


IQS SE - Undergraduate Program in Biotechnology