Estudi de microorganismes indicadors de contaminació fecal resistents a antibiòtics en aigües residuals


Sànchez Carbonell, Mireia  


Objectives: Antibiotic resistance is an increasingly important health problem. Its extensive and inappropriate use in various sectors has ended up leading to the infectivity of many of them, allowing the colonization and spread of resistant bacteria that result in a greater number of cases of serious infectious diseases difficult to treat. The aim of this work is to study the prevalence of antibiotic resistance (RA) of Escherichia coli and fecal coliforms (CF) in wastewater and to analyze which antibiotics develop greater resistance.

Methods: Two wastewater treatment plants (EDAR) located in the western Catalonia region were selected to extract wastewater samples. Chromocult selective medium was used to estimate the growth of E.coli and ampicillin-resistant fecal coliforms (AMP), ciprofloxacin (CPR), levofloxacin (LEV), ofloxacin (OFL), and sulfamethoxazole (SMX).

Results: A higher presence of ARB in E.coli was observed with respect to fecal coliforms. Percentages of fecal coliforms AR are significantly higher than outflows to inputs. High levels of ARB are obtained by AMP and CPR, and lower by LEV. An increase in ARB within EDAR is generated very prominent by LEV (more indicated by CF), and also by CPR by a EDAR.

Conclusions: E.coli AR is very present in wastewater at both inlets and outlets of EDARs, but EDARs appear to potentiate the increase in CF AR. This increase in ARB generated in fecal coliforms correlates with antibiotic concentration. The antibiotics with the most resistance are the oldest: AMP, CPR and SMX.

Keywords: Antibiotic-resistant bacteria, antibiotics, wastewater, Chromocult, Escherichia coli, fecal coliforms, EDAR.



Auset Vallejo, Maria 


IQS SE - Undergraduate Program in Pharmacy