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Author González Ríos, Nil |
Abstract Cancer disease, and especially lung cancer, has a high impact on today's society. The main causes that accentuate this disease are the lack of early diagnostic methods, as well as treatments with poor distribution throughout the body. All of this translates into severe side effects and a low survival rate. As an alternative, different studies have proposed immunotherapy, consisting of the selective activation of the immune system to fight malignant cells. Within the set of immunotherapies, it is intended to design a therapeutic vaccine based on nanoparticles. This vaccine must consist of an active principle that will be the mRNA encoding tumor-associated antigens, a vector capable of transporting and protecting the genetic material, and also surface ligands that allow vectorization to be carried out. In this way, when the vaccine is injected, it will be able to travel to the specific cells, transfect them and generate a specific response of the immune system against the cells that express this tumor antigen. In this work, a synthetic route capable of synthesizing ligands that allows the nano-structures to be directed towards a specific cell type has been carried out and fine-tuned. The design of this route starts from the use of a polymer, a poly (β-amino ester) - pBAE, capable of electrostatically interacting with the genetic material to form nanoparticles. By carrying out a functionalization of the polymer with mannose oligosaccharides, a vectorization of the nanosystem towards type C lectin receptors is allowed, which are over-expressed in dendritic cells, thus allowing the activation of the immune system. |
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Director Faijes Simona, Magda |
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Degree IQS SE - Master’s Degree in Bioengineering |
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Date 2021-05-06
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