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Author
Artamonov, Alexander
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Abstract
The small extracellular vesicles are extracellular vesicles that are implicated in cell communication and the transmission information as stimulus response or about certain states of the disease affecting the body. As the small extracellular vesicles are being attributed the role of transport of a variety of different molecules, ranging from proteins and lipids to mRNA and DNA. That said, it could be possible for the small extracellular vesicles to be used as drug delivery agents and assist in treatment of different diseases, such as cancer, cardiovascular diseases and tumors.
One of the main challenges of small extracellular vesicle manufacturing consists on the fact that they depend on the ability of production of large quantities in cells keeping their phenotype without being affected or altered. Current technologies such as 2D expansion platforms have certain limitations and therefore, potential research on technologies that involve maximizing surface area per ml of media, such as microcarriers, is underway.
This works aims to explore different techniques and materials used for various steps of cell production, such (I) cell count techniques, (II) cell harvesting techniques, (III) microcarriers characteristics and (III) cell and carriers’ re-utilization for cell-growth expansion and posterior scale up. All these procedures would help developing and optimizing a bioprocess of large-scale cells production and later small extracellular vesicles isolation, purification and modification.
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