Studying nuclear shuttling processes of AGO proteins in C. elegans


Rocamora Budet, Roberto  


Small RNAs (sRNA) have emerged as key regulators of gene expression in species eukaryotes. They are essential during development, responses to stress, for the preservation of the integrity of the genome and for immunity against viruses. To accomplish with their biological function, all Srna are loaded in Argonaute proteins (AGO), which they recognize and negatively regulate "target" genes. Depending on the organism and the pathway of Srna involved, AGO proteins can have nuclear and / or cytoplasmic localization. In the last years, research in the field of RNA silencing has focused primarily on the localization subcellular AGO. However, the import / export routes, as well as the transitory location they still remain practically unknown in all eukaryotic organisms. This has limited our knowledge of the specific subcellular functions of AGO proteins and our understanding the mechanisms of intracellular movements of sRNA.This work focuses on the development of GFP-AGO reporter vectors and the subsequent generation and characterization of transgenic worms to understand the transport mechanism of the HRDE1 protein and its subcellular localization to the Caenorhabditis model organism elegans.



Faijes Simona, Magda 
Bologna, Nicolas  


IQS SE - Undergraduate Program in Biotechnology