Caracterització, purificació i quantificació d'anticòs IgG1


Escala López, Laia 


Genetic engineering has made it possible to create universal binding molecules with high specificity for their target, such as monoclonal antibodies (MABS), considered indispensable tools in research, diagnosis and therapy. The growth of therapeutic MABS is evident, as they have become the predominant treatment modality for a wide range of diseases during the last 25 years. The great technological advances have allowed the development and discovery of new, more efficient and faster therapies, giving rise to a very competitive sector. All of this must be balanced against the reduction in the number of side effects and the overall economic cost. In the MABS production process, optimization of the upstream and downstream processes is very important to improve performance and minimize costs. The purpose of this study is to compare the growth of a murine hybridoma in two CD and SFM culture media to evaluate the efficacy of each of them. Glucose and lactate concentration is analyzed along growth kinetics to study cell metabolism. The SFM medium has been found to be optimal for the growth of the 1-EAE hybridoma compared to the CD medium. Finally, protein G affinity chromatography purification has proven to be an efficient method to quantify the IgG1 protein of interest.

Keywords: monoclonal antibodies, IgG, hybridoma, growth kinetics, culture medium, affinity chromatography, purification.



Lecina Veciana, Martí  


IQS SE - Undergraduate Program in Pharmacy