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Author Möller Ribas, Eduard |
Abstract Rhamnolipids are molecules of interest for their potential as biosurfactants, and for their rapid biodegradability in the environment. The basis of this work is to contribute to the creation of a heterologous system for the production of rhamnolipids, since the known homologous system is Pseudomonas aeruginosa, a pathogenic bacterium. The microalgae Chlamydomonas reinhardtii is chosen for its growth and production capacity, but it does not have the rhlA and rhlB genes that are responsible for the synthesis of rhamnolipids. Apart from this, the bioavailability of the rhamnolipid precursors is not fully known, and therefore, the objective of the work is to explore sequence and bibliographic databases to find information on the bioavailability of the dTDP-L-rhamnose precursor, in the organism C. reinhardtii. Due to the paucity of information on C. reinhardtii, information is sought on a model organism of plant systems, Arabidopsis thaliana. Information is sought in bioinformatic resources such as databases, subcellular location prediction tools, homologue search engines and sequence alignment programs, with the intention of finding if the enzymes that generate the metabolite of interest are present and if so, in which part of the cell. The homologous sequences of the dTDP-L-rhamnose biosynthesis pathway from other plant organisms and organisms related to the pathway are compared, and it is found that there are important well-conserved residues that are also present in some C. reinhardtii enzymes. Therefore, there is evidence that C. reinhardtii is ready for the biosynthesis of dTDP-L-rhamnose. |
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Director Leivar Rico, Pablo |
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Degree IQS SE - Undergraduate Program in Biotechnology |
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Date 2020-09-08
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