Modulación diferencial de los neuroquímicos monoaminérgicos centrales y periféricos por deprenyl en larvas de pez cebra


Bartolomé Alonso, Helena


Zebrafish embryos and larvae are vertebrate models increasingly used in translational neuroscience research. To link behavioral changes with alterations in the profile of NTs in the brain in these models, different analytical methods have been developed to determine the monoaminergic system in these organisms. In all the developed methods, whole larvae were used, raising the question of whether the whole larvae study model is representative of changes in the monoaminergic profile in the brain. For this reason, in this study, the levels of 10 monoaminergic NTs have been determined in the head, trunk, and whole body of zebrafish larvae. It has been found that the majority of NTs studied occur in a higher proportion in the head than in the trunk, or at similar levels in some cases (tyrosine and 5-HIAA). In addition, zebrafish larvae have been exposed to deprenyl, a drug used to treat Parkinson's and depression that is characterized as a prototypical inhibitor of monoamine oxidase, to evaluate the effect on organisms. In this treatment, it has been observed that significant changes occur in the distribution of some neurochemicals in the different parts of the larval body after treatment with deprenyl. While deprenyl increases serotonin and norepinephrine in both the head and trunk, the levels of dopamine, L-DOPA, and homonylic acid are only modulated in the head. Furthermore, the very significant increase in dopamine levels observed in the head after deprenyl treatment was not detected in the analysis of the whole larvae.
These results indicate that the analysis of NTs in zebrafish larvae should not be used as a general substitute for brain-specific analysis, as it may mask changes in the profiles of NTs.



Gómez Canela, Cristian


IQS SE - Master’s Degree in Analytical Chemistry