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Author
Mayol Cabré, Marta
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Abstract
Monoaminergic neurotransmitters are the main components that regulate a lot of processes in the vertebrate brain. There is a growing interest to monitor the changes produced in these neurochemicals due to the large number of exogenous agents such as pharmaceuticals, drugs of abuse and environmental pollutants, targeting and affecting
this system. Adult zebrafish (Danio rerio) shares the common neurotransmitter pathways and nervous system organization with mammals, and has been increasingly used as a research model in the last decades. Therefore, a method based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed for the first time to determine the profile of ten monoaminergic neurochemicals in the main brain areas of adult zebrafish (forebrain, midbrain and hindbrain). The applied analytical method has been studied in terms of quality such as linearity, sensitivity and intra- and inter-day precision. Subsequently, it has also been applied to determine changes on the neurotransmitters profile after the exposure of adult zebrafish to environmental relevant concentrations of glyphosate for two weeks. After the analysis of samples, significant differences on the levels of these neurotransmitters have been found between forebrain, midbrain and hindbrain, as well as after the exposure to glyphosate. Finally, a LC-MS/MS method has also been developed to determine glyphosate in water.
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