Optimización de la producción de anticuerpos monoclonales en un Wave Biorreactor


Martínez Cadenas, Rodrigo


The present work has been carried out in the cabs custom antibody development service. The platform has a BioBundle bioreactor system, the objective of which will be integrated as an additional service to the platform. To this end, it is important to fine-tune the system, which entails knowing each stage of the mid-scale monoclonal antibody production process. This begins with the introduction to the world of antibodies, within which we find Immunoglobulins G, the protagonists of this work. Immunoglobulins G are produced by B lymphocytes as a response of the humoral immune system. For the production of monoclonal antibodies, lymphocyte cells from animals previously immunized with the antigen of interest are used. Since this cell line does not divide indefinitely, they must fuse with myeloma cells to give rise to the producer cell line, the hybridomes.The hybridomas will finally be cultured in a Wave bioreactor, but in order to do so, the line must first be characterized. through parameters such as growth rate, glucose and glutamine consumption, and lactate, ammonium, and antibody production. That is why the choice of culture medium plays a crucial role, which must adapt to the metabolism of the hybridoma, minimizing the lactate and ammonium production rate and maximizing both cell growth and antibody production. Once this has been achieved, the decision focuses on the mode of operation, within which the conclusion is reached that the Fed-Batch is the one that best adapts to this production system, finally simulating what cell growth would be like in some feeding conditions limiting glucose and glutamine.



Lecina Veciana, Martí
Pascual, Núria


IQS SE - Undergraduate Program in Biotechnology