Síntesis de "single enzyme nanoparticles" para la construcción de un biosensor electroquímico


Conde Mendoza, Santiago


Electrochemical biosensors are analytical devices that combine the specificity of biochemical reactions with the analytical capabilities of electrochemical techniques. Due to this combination, it is possible to determine different analytes quickly, sensitively and reliably in samples with complex matrices. For all these reasons, the use of biosensors is an alternative to classical analytical methods for quality control processes in various industrial sectors.
Biosensors require a specific recognition system or bioreceptor. The instability of these elements, particularly enzymes, limits their use when building detection systems. For the biosensor to be effective, the biorecognition elements, and in this case the enzymes, must be immobilized on the surface of the electrochemical interface to ensure that they are fixed. In the present project, biosensors have been developed based on an enzyme encapsulation method with acrylamide gel that allows the immobilization of enzymes at the electrochemical interface also increasing their stability. In turn, different characterization techniques have been used to study the encapsulated enzyme and the performance of the constructed biosensors.
Finally, the performance of these biosensors has been compared with native enzyme biosensors, obtaining better results of stability and reproducibility for the proposed biosensors.



Colominas Fuster, Sergi
Abellà Iglesias, Jordi


IQS SE - Master’s Degree in Analytical Chemistry