Serotype-specific detection of dengue viruses in a nonstructural protein 1-based enzyme-linked immunosorbent assay validated with a multinational cohort


Bosch, Irene; Reddy, Ankita; de Puig, Helena; Ludert, Juan E.; Perdomo-Celis, Federico; Narváez, Calos F.; Versiani, Alice; Fandos, Diana; Nogueira, Mauricio L.; Singla, Mohit; Lodha, Rakesh; Medigeshi, Guruprasad R.; Lorenzana, Ivette; Ralde, Hugo Vicente; Gelvez-Ramirez, Margarita; Villar, Luis A.; Hiley, Megan; Mendoza, Laura; Salcedo, Nol; Herrera, Bobby Brooke; Gehrke, Lee


Dengue virus (DENV) infection is an increasingly significant threat to global health, with a yearly estimate of 390 million infections and an expected increasing burden with the rise of climate change and globalization. DENV is caused by one of the four serotypes (DENV-1-4), each of which have been associated with different immune responses and clinical manifestations. We developed a method to detect DENV serotypes by targeting the nonstructural 1 (NS1) antigen through an enzyme-linked immunosorbent-based assay (ELISA) with high sensitivity and specificity. We demonstrate that our high throughput mouse-derived antibody screening method selected for optimal test performance. The antibodies were integrated into an ELISA that can distinguish between the four different dengue serotypes by serotype-specific pairing. In addition, we provide a dengue universal antibody combination that enables pan-virus detection independently of the serotype. We use the ELISA in three different countries and calculate overall and site-specific sensitivities and specificities. The assay performs optimally when levels of viremia are high during the first five days of fever.








Plos Neglected Tropical Diseases, 24 June 2020, v.14, n.6, e0008203

Data de publicació